Journal: Cancer Biology & Medicine
Article Title: Quantitative proteomic analysis for high-throughput screening of differential glycoproteins in hepatocellular carcinoma serum
doi: 10.7497/j.issn.2095-3941.2015.0010
Figure Lengend Snippet: Quantitative proteomic schematic of serum glycoproteins. Glycoproteins in serum samples were enriched using lectin affinity chromatography and digested by trypsin then labeled using isotopic formaldehyde, followed by 2D RP-RP LC-MS/MS.
Article Snippet: Lectin affinity columns were prepared by adding 400 μL each of Con A and WGA slurry to empty Micro Bio-Spin columns (Bio-Rad Laboratories, Hercules, CA), as reported by Wei et al . . Con A exhibited a high affinity to high-mannose type N-glycans, whereas WGA was selective for N-acetyl-glucosamine (GlcNAc).
Techniques: Affinity Chromatography, Labeling, Reversed-phase Chromatography, Tandem Mass Spectroscopy